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Category:1787 births
Category:1848 deaths
Category:Bishops of the Church of England
Category:18th-century English Anglican priests
Category:19th-century English Anglican priests
Category:People educated at Cranmer Hall, Durham
Category:People from Durham, EnglandMass spectrometry (MS) is a powerful analytical tool for analyzing biomolecules. MS provides a means of detecting and characterizing a wide range of chemical species, including metabolites, lipids, proteins, peptides, and oligosaccharides. In addition, the sensitivity of MS makes it particularly useful for measuring the concentrations of substances, e.g., drugs, in biological fluids, such as blood or plasma. Mass spectrometers may be used in a wide range of applications, including: proteomics, metabolomics, drug discovery and development, biomarker discovery, and clinical diagnostics.
Desorption electrospray ionization (DESI) is a desorption ionization technique that incorporates electrospray ionization (ESI) with contactless desorption. DESI, in contrast to most other liquid desorption techniques, operates without any solvent flow. The sample is simply deposited onto the surface of a probe tip and a voltage is applied to the probe tip. This causes analyte ions to be desorbed into the gas phase where they are ionized by the impact of electrospray.
In a DESI instrument, a probe tip is typically mounted on the back end of a liquid-transfer pipette for liquid sample deposition. The probe tip is usually enclosed in an electrostatic chuck, which limits the sample to be deposited to only the portion of the tip that comes into contact with the chuck surface. The portion of the probe tip that is outside of the electrostatic chuck surface is known as the sample “reservoir.” The sample is desorbed electrospray ionization by applying a voltage to the tip. At the same time, the applied voltage creates an electric field between the probe tip and the surface of the chuck, which attracts the analyte sample from the reservoir to the sample ejection orifice of the probe tip. As a result, the sample molecules can be deposited on the surface of the probe tip in the vicinity of the orifice.
Probe tips for use in a mass spect ac619d1d87
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